| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 2 | HEISS, Presley | FR | Nebraska-Kearney | 26.24 |
| 3 | JEWELL, Katie | FR | UC-Colo. Spgs. | 24.83 |
| 4 | BULLOCK, Zane | JR | UC-Colo. Spgs. | 24.37 |
| 5 | FETTIG, Taryn | JR | UC-Colo. Spgs. | 24.45 |
| 6 | LAUGHLIN, Jadah | SO | Nebraska Wesleyan | 25.35 |
| 7 | SCHMIDT, Koral | JR | Nebraska-Kearney | 26.47 |
| 8 | WILLIAMS, Grace | SO | Nebraska-Kearney | 26.93 |