| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Valentine | A | Larsen | Buechle | Gillette | Lancaster | 3:50.00 | |
| 2 | Cozad | A | Snider | Davis | Snider | Hoffmaster | 3:42.31 | |
| 3 | Ogallala | A | Castillo | Gager | Rezac | Bush | 3:41.23 | |
| 4 | McCook | A | Walgren | Gross | Wilkinson | Gomez-Wilson | 3:31.67 | |
| 5 | Gothenburg | A | Harbur | Burge | Davis | Geiken | 3:31.73 | |
| 6 | Minden | A | Brais | Gibb | Boudreau | Holsten | 3:42.07 | |
| 7 | Broken Bow | A | Neely | Oeltjen | Harrold | Moniger | 3:45.18 |