| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Broken Bow | A | Flores | Wells | Schmidt | Markham | 48.45 | |
| 2 | Valentine | A | Gillette | Perrett | Larsen | Kreutner | 46.45 | |
| 3 | Cozad | A | White | Chytka | Irish | Chytka | 44.89 | |
| 4 | McCook | A | Fraker | Dugger | Gomez-Wilson | Schmidt | 43.85 | |
| 5 | Gothenburg | A | Harbur | Stevens | Burge | Geiken | 44.50 | |
| 6 | Ogallala | A | Brewer | Curtis | Bush | Rezac | 45.83 | |
| 7 | Minden | A | Brais | Gibb | Glanzer | Holsten | 46.57 |