| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KERRIGAN, Katherine | JR | Ainsworth | 5:28.21 | |
| 2 | HENNING, Lindee | JR | Ogallala | 5:39.82 | |
| 3 | APPLEGATE, Mallory | SR | Cozad | 5:42.46 | |
| 4 | RENNER, Isabella [Izzy] | JR | McCook | 5:45.25 | |
| 5 | CAPPEL, Emma | FR | McCook | 5:45.93 | |
| 6 | HURT, Jessie | SR | Minden | 5:49.97 | |
| 7 | KENNEDY, Emma | SO | Ainsworth | 5:53.09 | |
| 8 | TOBEY, MaKinley | JR | Broken Bow | 5:54.98 | |
| 9 | RODEWALD, Samantha | SR | McCook | 5:59.38 | |
| 10 | HENNING, Lauren | JR | Ogallala | 6:00.96 | |
| 11 | WEIDES, Lorna | SR | Holdrege | 6:03.00 | |
| 12 | STAPLES, Gaby | SO | Broken Bow | 6:03.05 | |
| 13 | STAPLES, Jaycee | FR | Broken Bow | 6:09.93 | |
| 14 | NEGLEY, Abby | FR | Gothenburg | 6:29.16 | |
| 15 | SALOMON, Avery | SO | Gothenburg | 6:30.34 | |
| 16 | PORTER, Ayleigh | JR | Minden | 6:44.43 | |
| 17 | INIGUEZ-JAUREGUI, Alejandra | SR | Minden | 6:47.60 | |
| 18 | SPRENGER, Samantha | FR | Valentine | 6:47.94 | |
| 19 | KOMENDA, Kelsey | JR | Holdrege | 6:56.35 | |
| 20 | BARNES, Haley | JR | Cozad | 6:57.65 | |
| 21 | ALBRECHT, Ellie | JR | Cozad | 6:58.73 |