| N | NAIA Qualifying | 53.70 |
| P | NCAA Provisional | 53.32 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BENSON, Ryan | FR | South Dakota St. |
57.19 | 16th Annual Wayne State - Wildcat Classic |
| 2 | BERTRAND, Lane | JR | Nebraska-Kearney |
55.83 | 2022 Prairie Wolf Invite |
| 3 | KREJCI, John | JR | Doane |
55.22 | 2022 GPAC Championships |
| 4 | DRAHOTA, Jack | JR | Nebraska-Kearney |
53.19 | 2022 Loper Twilight |
| 5 | CLAUSEN, Brian | SR | Hastings |
54.05 | 2022 GPAC Championships |
| 6 | HARTZLER, Connor | SO | Hastings |
55.58 | 2022 Concordia Twilight |
| 7 | BELDEN, Jarod | JR | York (Neb.) |
56.72 | KCAC Outdoor Track & Field Championships |
| 8 | SALMANS, Ethan | SO | Fort Hays St. |
57.49 | Emporia State Relays |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | VARGAS, Gunner | SO | Northwestern (Iowa) |
1:02.85 | South Dakota Challenge |
| 2 | GONZALEZ, Jayden | JR | Hastings |
1:02.23 | 2022 Prairie Wolf Invite |
| 3 | FRIES, Gage | FR | Concordia (Neb.) |
59.50 | |
| 4 | FARMER, Kameron | JR | Concordia (Neb.) |
59.22 | 2022 Concordia Invite |
| 5 | REMSBERG, Jonah | FR | Fort Hays St. |
59.39 | Emporia State Relays |
| 6 | MEYER, Teagan | SO | Concordia (Neb.) |
1:00.11 | 2022 GPAC Championships |
| 7 | VAN ZEE, Reese | FR | Northwestern (Iowa) |
1:02.83 | 16th Annual Wayne State - Wildcat Classic |
| 8 | BOMGAARS, AJ | FR | Northwestern (Iowa) |
1:05.94 | 16th Annual Wayne State - Wildcat Classic |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HOSKINS, Abraham | FR | Nebraska-Kearney |
NT | |
| 4 | FRANDOLIG, Zachary | SO | Nebraska Wesleyan |
NT | |
| 5 | SAGEHORN, Ashton | FR | Doane |
NT |