| Lane | Athlete | Team | Seed | 
|---|---|---|---|
| 3 | DUVERGER, Misha | East Stroudsburg | NT | 
| 4 | QUIST, Bethany | Bryn Mawr | 1:07.01 | 
| 5 | RHODES, Jalen | East Stroudsburg | 1:07.87 | 
| Lane | Athlete | Team | Seed | 
|---|---|---|---|
| 2 | FRATANDUONO, Anastasia | Kutztown | 1:04.00 | 
| 3 | BENDETTI, Ava | Kutztown | 1:02.00 | 
| 4 | BONNER, Raechel | Kutztown | 56.96 | 
| 6 | AMIN, Alexandra | West Chester | 1:03.85 | 
| 7 | MAYERS, Kiara | Kutztown | 1:04.34 | 
| 8 | KELLY, Rachael | Kutztown | 1:06.97 |