| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 35 | SINGLETON, Kolden | SO | Cherokee High School | 46.85 | Verified |
| 2 | 10 | HAYES, Lofton | 9 | Cherokee High School | 46.00 | Unverified: |
| 3 | 116 | ROSS, John | JR | Volunteer High School | 43.86 | Verified |
| 4 | 115 | WINEGAR-COLLIS, Jordyn | SO | Volunteer High School | 45.90 | Verified |
| 5 | 34 | SMITH, Trey | JR | Cherokee High School | 46.12 | Verified |
| 6 | 63 | WOODS, Sam | SR | Providence Academy | 52.00 | Verified |