| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 5 | E.C. Glass | A | Harrington | Wilson | Hull | Patterson | 4:55.61 | Verified |
| 6 | Orange County | A | Winn | Cropp | Testa | Urgeles Montaner | 4:56.47 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Salem (Roanoke) | A | Murphy | Bruno-Gaston | Mangus | Armistead | 4:47.86 | Verified |
| 3 | Amherst County | A | Taylor | Chapman | Smith | Lombard | 4:28.96 | Verified |
| 4 | Louisa County High School | A | David | Middlesworth | Myers | Ryan | 4:18.66 | Verified |
| 5 | Western Albemarle | A | 4:11.35 | Verified | ||||
| 6 | Blacksburg High School | A | Bowman | Krouscas | Kehlenbeck | Farley | 4:13.63 | Verified |