| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Happy Valley High School | A | Paul | Walker | Pate | Deakins | 1:51.59 | Verified |
| 4 | Elizabethton High School | A | 1:41.10 | Verified | ||||
| 5 | Unicoi Co. High School | B | 1:49.44 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | University School of Johnson Cit | A | 1:40.71 | Verified | ||||
| 3 | South Greene High School | A | DNS | Verified | ||||
| 4 | Chuckey-Doak High School | A | 1:34.82 | Verified | ||||
| 5 | Tennessee High School | A | Johnson | Green | Harkleroad | Hernton | 1:35.19 | Verified |
| 6 | Volunteer High School | A | 1:40.59 | Verified | ||||
| 7 | Sullivan East High School | A | 1:40.81 | Verified |