| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Sullivan East Middle School | A | Hewitt | Dickenson | Osborne | Brewer | 4:33.88 | Verified |
| 2 | John Sevier Middle School | A | Williams | Tate | Fields | Freeman | 4:20.13 | Verified |
| 3 | Church Hill Middle School | A | Begley | Hall | Jennings | Barker | 4:28.33 | Verified |
| 4 | Sullivan Heights Middle School | A | Lyons | Henopp | Davidson | Lawson | 4:43.13 | Verified |