| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | T A Dugger Junior High School | A | Holsclaw | Everette | Miller | Wilber | 2:11.66 | Verified |
| 4 | Sullivan Heights Middle School | A | Deal | Peake | Garcia | Carpenter | 2:08.95 | Verified |
| 5 | John Sevier Middle School | A | Cradic | Lawson | Smith | McReynolds | DNS | Verified |
| 6 | Church Hill Middle School | A | 2:07.15 | Verified | ||||
| 7 | Bristol Tennessee Middle School | A | Manahan | Quillen | Slagle | Smith | 2:09.87 | Verified |
| 8 | Sullivan East Middle School | A | Barrett | Widner | Patrick | Giles | 2:11.84 | Verified |