| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | John S. Battle | A | Poe | Moore | DeRaps | Ratliff | DNS |
| 3 | Tazewell | A | 2:12.23 | ||||
| 4 | Virginia High School | A | 2:15.56 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Chilhowie | A | 2:03.30 | ||||
| 3 | Floyd County | A | DNS | ||||
| 4 | Abingdon High School | A | Agner | Boyd | Mays | Odum | 2:00.73 |
| 5 | Auburn | A | Connelly | Smith | Spencer | Sale | 2:08.00 |