| Lane | # | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|---|
| 1 | 72 | SOMERVELL, Tess | JR | Chilhowie | 2:26.97 |
| 2 | 73 | PAYAN, Bella | SO | Chilhowie | 2:51.71 |
| 3 | 45 | RUSSELL, Averi | SO | Lebanon | 2:52.80 |
| 4 | 126 | LAUDIE, Amaura | JR | Patrick Henry (G. Spring) | 2:57.14 |
| 5 | 74 | BLACKWELL, Tori | 9 | Chilhowie | 3:00.21 |
| 6 | 127 | COOK, Lexi | SO | Patrick Henry (G. Spring) | 3:01.75 |
| 7 | 75 | FRYE, Presley | SO | Chilhowie | 3:07.13 |
| 8 | 76 | GROSS, Kendyl | 9 | Chilhowie | 3:08.97 |
| 9 | 32 | BURKS, Serenity | 9 | Holston | 3:12.06 |
| 10 | 27 | MILHORNE, Maggie | SR | Holston | 3:22.63 |
| 11 | 28 | HERNDON, Grace | 9 | Holston | 3:32.49 |
| 12 | 8 | PHILLIPS, Shaylee | 9 | Northwood | 3:35.69 |
| 13 | 9 | KESTNER, Liz | JR | Northwood | 3:52.21 |
| 14 | 52 | DILLON, Chloee | 9 | Lebanon | NT |
| 15 | 42 | STEELE, Gabbie | JR | Lebanon | NT |