| TN | Times News Relays | 2:36.00 |
| Lane | Athlete | Yr. | Team | Seed |
|---|---|---|---|---|
| 1 | JESSEE, Makaleigh | JR | Abingdon High School | 2:23.78 |
| 2 | JOHNSON, Cecelia | JR | Abingdon High School | 2:50.38 |
| 3 | LOONEY, Jessi | JR | Grundy | 2:51.27 |
| 4 | DICK, Katie | SO | Patrick Henry (G. Spring) | 2:56.17 |
| 5 | BURKS, Annina | 9 | Holston | 3:00.47 |
| 6 | LAMBERT, Caitlin | JR | John S. Battle | 3:03.40 |
| 7 | WOODARD, Kirsten | SO | Patrick Henry (G. Spring) | 3:06.05 |
| 8 | WHEATLEY, Katelyn | SO | Carroll County | 3:07.24 |
| 9 | INGLE, Chloe | SO | John I. Burton | 3:11.13 |
| 10 | MILHORNE, Maggie | JR | Holston | 3:21.43 |
| 11 | FRYE, Presley | 9 | Chilhowie | 3:29.77 |
| 12 | CONNER, Aaliyah | 9 | Bluefield | 3:31.45 |
| 13 | MIXON, Ashley | SR | Chilhowie | 3:36.93 |
| 14 | VENCILL, Heileigh | JR | Grundy | NT |
| 15 | GREEAR, Jadah | JR | Castlewood | NT |
| 16 | MORRISON, Jillian | JR | John S. Battle | NT |
| 17 | CRUZ, Nahirana | SO | Carroll County | NT |
| 18 | JARVIS, Kendall | SO | John S. Battle | NT |