| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Patrick Henry (G. Spring) | A | Clay | Maiden | Steele | McKinney | NT |
| 2 | John S. Battle | A | Ratliff | Gardner | Little | Bishop | 59.99 |
| 3 | George Wythe (Wytheville) | A | 56.48 | ||||
| 4 | Bluefield | A | Dowell | Padgett | Robinson-Lee | Hodge | DQ |
| 5 | Marion | A | Whitt | Whitt | Hagy | Umbarger | DNS |
| 6 | Abingdon High School | A | Andis | Blackmon | McConnell | Whitman | NT |