| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Copperhead Track | A | Hawkins | Ricketts | Boatcher-Wendt | Shipwash | NT |
| 2 | Holston | A | Tweed | Tweed | Leonard | Richardson | NT |
| 3 | Copperhead Track | B | Maxwell | Taylor | Hanson | Kerns | NT |
| 4 | Abingdon High School | C | Ratliff | Ramirez | Ratliff | Bowers | NT |
| 5 | Abingdon High School | A | Stanley | Buchanan | Muncy | Johnson | NT |
| 6 | Abingdon High School | B | Molinary | Stanley | Thompson | Bartley | NT |