| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Cannon | A | Bryan | Biehler | Trembath | Pridgen | NT |
| 4 | Providence Day | A | Hughes | Latorre | Artis | Haney | 2:17.00 |
| 5 | Charlotte Latin | A | Fletcher | Casey | Bradley | Alexander | NT |
| 6 | Charlotte Country Day | A | Miller | Schulte | Fonville | McMichael | NT |