| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Charlotte Latin | A | Ngwanza | Little | Provencher | Guggenheimer | DNS |
| 3 | Charlotte Country Day | A | Utley | Deets | Macklin | Workinger | NT |
| 4 | Providence Day | A | Doffermyre | Winston | Mendoza | Ray | 1:47.00 |
| 5 | Covenant Day | A | Rivens | Broadway | Fitch | Keel | NT |
| 6 | Cannon | A | Otey | Kellman | Levy | Clarke | NT |