| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Cannon | A | Stradford | Springate | Ristich | Morocco | NT |
| 3 | Charlotte Latin | A | Maxwell | Thompson | Wheeler | Haines | NT |
| 4 | Charlotte Country Day | A | Stevens | Macklin | Johnson | Riley | 1:40.00 |
| 5 | Covenant Day | A | Collins | Dennison | Broadway | Wright | 1:52.00 |
| 6 | Providence Day | A | Stuart | Oriaku | Scott | Smith | NT |