| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Charlotte Latin | A | Klingman | Guggenheimer | Smith | DiRoma | NT |
| 2 | Charlotte Country Day | B | Wilkerson | Pham | Cox | Yuhas | 12:15.00 |
| 3 | Providence Day | A | Roemer | Harris II | Michaelson | Makemson | NT |
| 4 | Charlotte Country Day | A | Pignetti | West | Norton | Kleiderer | 10:50.00 |
| 5 | Providence Day | B | Cronk | Thomas | Desollar | Hoody | NT |
| 6 | Covenant Day | A | DeYoung | Burns | Duncan | DeYoung | NT |
| 7 | Cannon | A | Boon | Wheeler | Finn | Friedli | NT |