| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Cannon | A | Bryan | Biehler | Trembath | Pridgen | NT |
| 3 | Charlotte Country Day | A | Miller | McMichael | Schulte | Erwin | NT |
| 4 | Providence Day | A | Eziri | Brainard | Goddard | Spearman | DNS |
| 5 | Covenant Day | A | Patton | Jordan | Thomas | Mabry | 1:03.00 |
| 6 | Charlotte Latin | A | Stradford | Kim | Green | Lavelle | NT |