| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Cannon | A | Woolwine | Hayworth | Kinsella | Fuller | NT |
| 3 | Providence Day | A | Abraham | Brooks | Diman | Ray | NT |
| 4 | Charlotte Country Day | A | Harrick | Mast | Anton | Workinger | 3:46.00 |
| 5 | Covenant Day | A | Cogdill | Joyce | Keel | Vandenberg | 3:48.00 |
| 6 | Charlotte Latin | A | Bershad | Wheeler | Ghirmay | Provencher | NT |