| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Richlands | A | Blankenship | Davis | Wall | Whited | DNS |
| 2 | Lee | B | 1:43.60 | ||||
| 3 | Abingdon High School | A | Stanley | Molinary | Thompson | Stanley | 1:35.07 |
| 4 | Abingdon High School | B | Bartley | Johnson | Ramirez | Miller | 1:35.07 |
| 5 | Lee | A | Early | May | Stafford | Grace | 1:43.60 |