| MR | Times News Relays | 42.17 | Dobyns-Bennett | 2001 |
| Lane | Team | Squad | Seed |
|---|---|---|---|
| 1 | Holston | A | 46.77 |
| 2 | Daniel Boone High School | A | 46.03 |
| 3 | West Ridge High School | A | DNS |
| 4 | Science Hill High School | A | DNS |
| 5 | Greeneville High School | A | 42.22 |
| 6 | Dobyns Bennett High School | A | 43.78 |
| 7 | Abingdon High School | A | DNS |
| 8 | Elizabethton High School | A | 45.75 |
| 9 | John I. Burton | A | 46.69 |