| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | ANDERSON, Savannah | Berea |
29.42 |
| 2 | KOROMA, Hannah | Berea |
28.41 |
| 3 | HAMPTON, Alexzandria | Berea |
27.26 |
| 4 | DYER, Ka'shauna | Carson-Newman |
26.28 |
| 5 | CURLEY, Elise | Carson-Newman |
26.49 |
| 6 | KING, Harmonee | Maryville (Tenn.) |
28.28 |
| 7 | PEREZ PENA, Oriana | Berea |
29.34 |
| 8 | DANI, Egzona | Berea |
31.24 |