| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Neumann |
A | 5:01.79 | 5:01.79 WCU Purple %26 Gold Open |
| 3 | Pratt Institute | A | 4:32.25 | 4:32.25 Pratt Invitational 2026 |
| 4 | Marywood |
A | 4:11.42 | 4:11.42 2026 Danny Curran Invitational |
| 5 | Immaculata |
A | 4:17.89 | 4:17.89 DeSales Quad Meet |
| 6 | Marymount (Va.) |
A | 4:20.43 | 4:20.43 21st Paul Kaiser Classic |
| 7 | St. Elizabeth | A | 4:51.47 | 4:51.47 Osprey Open |