| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Gwynedd-Mercy |
A | 4:04.76 | 4:04.76 Miller Sports Construction Blue Jay Tune-Up |
| 3 | Immaculata |
A | 3:35.90 | 3:35.90 2026 Misericordia Invitational |
| 4 | Marymount (Va.) |
A | 3:31.30 | 3:31.30 2026 Salisbury University Eastern Shore Challenge |
| 5 | Neumann |
A | 3:34.91 | 3:34.91 Delaware Invite |
| 6 | Marywood |
A | 3:35.20 | 3:35.20 2026 Misericordia Invitational |
| 7 | St. Elizabeth | A | 3:52.28 | 3:52.28 Osprey Open |