| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | St. Elizabeth | A | 44.93 | 44.93 Osprey Open |
| 4 | Marymount (Va.) |
A | 42.38 | 42.38 2026 George Mason Dalton Ebanks Invitational |
| 5 | Marywood |
A | 44.17 | 44.17 York College Twilight Meet |
| 6 | Neumann |
A | 44.27 | 44.27 Penn Relays |
| 7 | Immaculata |
A | 45.79 | 45.79 DeSales Quad Meet |