| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Pratt Institute | A | DQ | Vassar April Twilight |
| 3 | Gwynedd-Mercy |
A | 45.07 | Gwynedd Mercy Last Chance Invitational |
| 4 | Marywood |
A | 43.71 | 2025 Kings College Monarch Open |
| 5 | Marymount (Va.) |
A | 43.49 | Mason-Dixon Invitational |
| 6 | Immaculata |
A | DNF | Danny Curran Invite |