| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | Day, Alex | SR | Grainger High School | NT | Unverified: |
| 3 | Taylor, Jacob | SO | Claiborne High School | 28.48 | Unverified: Volunteer High School Invite |
| 4 | Vermillion, Kaden | JR | Grainger High School | 29.96 | Unverified: Cherokee Meet #3 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Phipps, Dion | 9 | Greeneville High School | 27.92 | Verified |
| 2 | Leach, Auston | 9 | Cherokee High School | 25.53 | Verified |
| 3 | Delvalle, Joniel | SO | Greeneville High School | 25.26 | Verified |
| 4 | Dockery, Tajuan | 9 | Cocke County High School | 25.32 | Verified |
| 5 | Olson, Ryan | JR | Claiborne High School | 26.86 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Humphries, Darien | SO | Greeneville High School | 24.84 | Verified |
| 2 | Jones, Cole | JR | Claiborne High School | 23.77 | Verified |
| 3 | Hayes, Lofton | 9 | Cherokee High School | 23.61 | Verified |
| 4 | Price, Daniel | 9 | Cocke County High School | 23.62 | Verified |
| 5 | Gonzalez, Jaydan | 9 | Cherokee High School | 24.74 | Verified |
| 6 | Sutton, Will | 9 | Cocke County High School | 25.17 | Verified |