| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Hanahan | A | Furse | Wilson | Bermudez | Mills | NT |
| 4 | Oceanside Collegiate Academy | B | Canon | Poland | Towns | Bertchtold | 54.00 |
| 5 | Cane Bay | A | Allen | Colen | Denson | Miyazaki | 55.00 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Waccamaw | A | Gunn | Fitzgerald | Gunn | Sitas | 54.00 |
| 2 | Woodland | A | Ferrell | Moorer | Grayton | Kitt | DQ |
| 3 | Ashley Ridge | A | Cleland | Jones | Knotts | Cunningham | 51.00 |
| 4 | Battery Creek | A | Tobin | Sanders | Wilson | Smith | DNS |
| 5 | Philip Simmons | A | Simpson | Watson | Allen | Pannell | 50.12 |
| 6 | Stratford | A | Nixon | Buckley | Arline | Weiters | DQ |
| 7 | Fort Dorchester | A | Brown | Webber | Dotch | Larkins | 51.74 |
| 8 | Oceanside Collegiate Academy | A | Donovan | Mosquera | Denton | Cosgrove | DNS |