| # | Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|---|
| 1 | 933 | ARNAUT, DASHA | JR | WESTERN CAROLINA | 54.75 |
| 2 | 53 | PARKER, ADAYSHA | SR | TIFFIN | 55.92 |
| 3 | 607 | HART, ASIA | SR | QUEENS (N.C.) | 56.04 |
| 4 | 1010 | HARDWICK, SAIDAH | FR | LENOIR-RHYNE | 56.80 |
| 5 | 219 | WILLIAMS, IMANI | WINSTON-SALEM ST. | 56.85 | |
| 6 | 225 | PORTER, VICTORIA | WINSTON-SALEM ST. | 57.95 | |
| 7 | 211 | BURTON, AALIYAH | WINSTON-SALEM ST. | 58.00 | |
| 8 | 52 | DOSS, MADISON | SO | TIFFIN | 58.44 |
| 9 | 535 | COVINGTON, JERRICA | FR | LINCOLN MEMORIAL | 58.70 |
| 10 | 567 | BARMORE, JASMINE | SO | ANDERSON (S.C.) | 58.78 |
| 11 | 218 | JOHNSON, KEYONA | WINSTON-SALEM ST. | 59.27 | |
| 12 | 501 | HONORABLE, NEYASHA | SO | KING | 59.61 |
| 13 | 805 | PARASKEVA, MADISON | SO | WOFFORD | 59.89 |
| 14 | 115 | JAKOBS, FRANZISKA | FR | HIGH POINT | 1:00.00 |
| 15 | 170 | BLACKWOOD, AUTUMN | FR | TUSCULUM | 1:00.25 |
| 16 | 532 | CRAWFORD, JORDAN | SO | LINCOLN MEMORIAL | 1:00.31 |
| 17 | 887 | BRYANT, D'AMANI | FR | WESTERN CAROLINA | 1:00.50 |
| 18 | 25 | GENERAL, THEA | JR | TIFFIN | 1:01.65 |
| 19 | 980 | ALSTON, DAKOTA | JR | LENOIR-RHYNE | 1:02.00 |
| 20 | 506 | SMITH, ISIS | FR | KING | 1:02.29 |
| 21 | 196 | PRUIETT, BAILEY | FR | TUSCULUM | 1:02.36 |
| 22 | 546 | RATLIFF, HANNA | FR | LINCOLN MEMORIAL | 1:03.00 |
| 23 | 908 | LITTLE, MIA | JR | WESTERN CAROLINA | 1:03.26 |
| 24 | 203 | MINUS, RAYGENE | JR | TUSCULUM | 1:03.36 |
| 25 | 55 | NORMAN, ZAIREEA | SO | TIFFIN | 1:03.69 |
| 26 | 552 | ZANDER, KATE | SO | LINCOLN MEMORIAL | 1:04.00 |
| 27 | 577 | EDWARDS, EMILEE | SO | ANDERSON (S.C.) | 1:04.00 |
| 28 | 817 | FAISON , PARKE | UNATTACHED | 1:04.31 | |
| 29 | 205 | FRANKLIN, MIKEL | WINSTON-SALEM ST. | 1:05.00 | |
| 30 | 120 | DARAKJY, COURTNEY | FR | HIGH POINT | 1:05.00 |
| 31 | 714 | FANNEY, SOMMER | JR | GUILFORD | 1:06.19 |
| 32 | 149 | WILSON, CORY | JR | HIGH POINT | 1:06.28 |
| 33 | 313 | MCCOY, MILLIE | FR | LEES-MCRAE | 1:06.35 |
| 34 | 179 | WILDMAN, DREW | FR | TUSCULUM | 1:06.89 |
| 35 | 494 | GRAHAM, RACHEL | FR | KING | 1:07.02 |
| 36 | 1131 | CANNON, CLAUDIA | FR | NEWBERRY | 1:07.60 |
| 37 | 191 | QUAY, ANGEL | FR | TUSCULUM | 1:09.00 |
| 38 | 696 | BROWN, SAMANTHA | FR | GUILFORD | 1:09.46 |
| 39 | 488 | MCCORMICK, AUDREY | SO | KING | 1:11.61 |
| 40 | 541 | TISCH, KAITLIN | SO | LINCOLN MEMORIAL | NT |
| 41 | 216 | ROWE, QJUANDA | WINSTON-SALEM ST. | NT | |
| 42 | 214 | JACKSON, QIZEAH | WINSTON-SALEM ST. | NT | |
| 43 | 11 | FRANCIS, MERCEDEEZ | FR | TIFFIN | NT |
| 44 | 209 | FOY, KAYLA | WINSTON-SALEM ST. | NT |