| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | Cress, Morgan | Franklin | 3:16.03 |
| 2 | Herron, Aspen | Pisgah | 3:20.0h |
| 3 | Maheswaran, Liliana | Shining Rock Classical Academy | 4:00.00 |
| 4 | Hernandez Bautista, Alma | Avery County | 4:01.85 |
| 5 | Frizzell, Lilly | Swain County | NT |
| 6 | Gibson, Jaden | Shining Rock Classical Academy | NT |
| 7 | Hurtado, Josie | Avery County | NT |
| 8 | Plemmons, Cheyenne | Franklin | NT |
| 9 | Radecki, Molly | Franklin | NT |
| 10 | Swimmer, Dvdaya | Cherokee | NT |
| 11 | Timan, Abigail | Franklin | NT |
| 12 | Vepsalainen , Liinu | Avery County | NT |