| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Highlands | A | DNS | Verified |
| 2 | Andrews | A | 56.04 | Verified |
| 3 | Hiwassee Dam | A | 54.40 | Verified |
| 4 | Murphy | A | 53.59 | Verified |
| 5 | Robbinsville | A | 53.84 | Unverified: |
| 6 | Swain County | A | 55.22 | Verified |
| 7 | Hayesville | A | 56.44 | Verified |
| 8 | Andrews | B | 1:04.04 | Unverified: |