| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | LAMB, Cole | SR | Smoky Mountain | 52.18 | Verified | 
| 2 | DILLS, Jared | SR | Hiwassee Dam | 53.15 | Verified | 
| 3 | WHITE, Paul | SO | Hayesville | 55.14 | Verified | 
| 4 | MILLER, Jamie | SR | Hayesville | 55.17 | Verified | 
| 5 | HARRIS, Hunter | 9 | Murphy | 55.99 | Verified | 
| 6 | PEEBLES, Rocky | SO | Cherokee | 56.74 | Verified | 
| 7 | LOSSIAH, Josiah | SR | Cherokee | 57.57 | Verified | 
| 8 | CASTILLO, Heinreg | JR | Andrews | 58.04 | Verified | 
| 9 | MOORE, Dalton | SO | Murphy | 58.34 | Verified | 
| 10 | KEENER, Drey | 9 | Robbinsville | 58.64 | Verified | 
| 11 | COLLINS, Nathan | 9 | Robbinsville | 59.34 | Verified | 
| 12 | VAUGHN, Cade | SO | Andrews | 59.94 | Verified | 
| 13 | ARKANSAS, Jordan | 9 | Cherokee | 1:00.64 | Verified | 
| 14 | JONES, Gaige | 9 | Swain County | 1:00.74 | Verified | 
| 15 | FRENCH, Ki | JR | Smoky Mountain | 1:01.34 | Verified | 
| 16 | WARD, Forest | SO | Smoky Mountain | 1:02.34 | Verified | 
| 17 | FLAWS, Gabriel | SO | Tri-County Early College | 1:03.94 | Verified | 
| 18 | COTTEN, Jayden | 9 | Murphy | 1:04.34 | Verified | 
| 19 | OLVERA, Jeffrey | 9 | Highlands | 1:04.94 | Verified | 
| 20 | WIMPEY, William | SO | Hayesville | 1:06.42 | Verified | 
| 21 | HORNBUCKLE, Josh | JR | Swain County | 1:07.40 | Verified | 
| 22 | SAMTON, Michael | 9 | Andrews | 1:08.24 | Verified | 
| 23 | HAMMOND, Shane | SO | Andrews | 1:09.53 | Verified | 
| 24 | BOBO, Adam | 9 | Murphy | 1:09.53 | Verified | 
| 25 | MELTON, Daniel | SO | Smoky Mountain | NT | Unverified: |