| Athlete | Yr. | Team | Seed | Note | |
|---|---|---|---|---|---|
| 1 | BOLYARD, Sydney | JR | Tri-County Early College | 5:56.70 | Verified | 
| 2 | DUNCAN, Rebecca | JR | Swain County | 6:00.10 | Verified | 
| 3 | BURCH, Caroline | 9 | Hayesville | 6:16.71 | Verified | 
| 4 | CRAYTON, Lucy | JR | Tri-County Early College | 6:17.80 | Verified | 
| 5 | SCIFERS, Katherine | SR | Smoky Mountain | 6:23.10 | Verified | 
| 6 | REYES, Rosa | SO | Cherokee | 6:25.00 | Verified | 
| 7 | HOGEBACK, Dakota | SO | Murphy | 6:25.90 | Verified | 
| 8 | MEYERS, Phynley | SO | Swain County | 6:29.70 | Verified | 
| 9 | ULANER, Emily | 9 | Swain County | 6:33.15 | Verified | 
| 10 | WORLEY, Madison | JR | Murphy | 6:35.20 | Verified | 
| 11 | THOMAS, Allison | SO | Hayesville | 6:35.68 | Verified | 
| 12 | KAISER, Makayla | SO | Hayesville | 6:44.43 | Verified | 
| 13 | STROUPE, Ashlyn | 9 | Murphy | 7:00.20 | Verified | 
| 14 | GRAY, Natalie | JR | Hayesville | 7:08.00 | Verified | 
| 15 | BARREIRO, Vivianna | JR | Hiwassee Dam | 7:17.00 | Verified |