| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | WINFIELD, Kayla | David Crockett High School | 1:01.69 |
| 3 | DABRESCIA, Linnea | Sullivan South High School | 57.29 |
| 4 | TOPPING, Mattie | Sullivan South High School | 54.76 |
| 5 | CHRISTIAN, Whitney | Volunteer High School | 47.77 |
| 6 | COOPER, Jenah | Cherokee High School | 53.25 |
| 7 | SCOTT, Halle | David Crockett High School | 55.51 |
| 8 | ROSS, Lauren | Volunteer High School | 1:01.54 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | CARDENAS, Lora | Volunteer High School | NT |
| 4 | STADLER, Addie | David Crockett High School | 1:02.04 |
| 5 | WINDSOR, Sadie | Cherokee High School | 1:03.90 |