| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GILES, KARA | Sullivan Central High School | 1:06.38 | Verified |
| 2 | FAIN, EMILY | Sullivan East High School | 1:04.32 | Verified |
| 3 | HENGER, AUBREE | Dobyns Bennett High School | 1:04.00 | Unverified: |
| 4 | HOBACK, LAIKEN | Sullivan Central High School | 1:02.46 | Verified |
| 5 | RUSSELL, HARPER | Cherokee High School | 1:00.75 | Verified |
| 6 | GLOVER, REBECCA | Sullivan East High School | 1:01.71 | Verified |
| 7 | ANDERSON, KATIE | Sullivan East High School | 1:03.97 | Verified |
| 9 | ADKINS, LESLEY | Sullivan East High School | 1:06.04 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BRISTOL, INJOI | Dobyns Bennett High School | 1:04.00 | Unverified: |
| 2 | FERGUSON, SHANNON | Johnson Co. High School | NT | Unverified: |
| 3 | BRAY, LAKEN | Cherokee High School | 1:14.30 | Verified |
| 4 | GIBSON, PAYTON | Volunteer High School | 1:07.00 | Unverified: |
| 5 | ALLISON, JESSICA | Volunteer High School | 1:06.56 | Verified |
| 6 | HAMILTON, SAVANNA | Volunteer High School | 1:06.80 | Unverified: |
| 7 | WALTERS, SAMANTHA | Volunteer High School | 1:09.37 | Verified |
| 8 | BOWERY, GRACE | Sullivan South High School | 1:16.06 | Verified |
| 9 | RADTKE, ASHLEY | Sullivan South High School | NT | Unverified: |