| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Cherokee High School | A | 51.44 | Unverified: |
| 3 | Sullivan South High School | A | 48.77 | Verified |
| 4 | Volunteer High School | A | 46.29 | Verified |
| 5 | Happy Valley High School | A | 47.89 | Verified |
| 6 | Sullivan East High School | A | 49.20 | Unverified: |
| 7 | Johnson Co. High School | A | NT | Unverified: |