| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MAYS, TYLER | Dobyns Bennett High School | 2:03.63 | Verified |
| 2 | BLYTHE, GREYSON | Dobyns Bennett High School | 2:14.53 | Verified |
| 3 | FADIAL, HUNTER | Sullivan East High School | 2:14.58 | Verified |
| 4 | COBB, GABRIEL | Dobyns Bennett High School | 2:15.01 | Verified |
| 5 | NUNLEY, BRANDON | Sullivan Central High School | 2:15.49 | Verified |
| 6 | VAUGHN, CALEB | Sullivan South High School | 2:15.85 | Verified |
| 7 | MILLER, AUSTIN | Dobyns Bennett High School | 2:16.21 | Verified |
| 8 | SANDERS, MASON | Sullivan Central High School | 2:20.46 | Verified |
| 9 | BROOKS, HENRY | Cherokee High School | 2:21.59 | Verified |
| 10 | AMBURN, CALEB | Dobyns Bennett High School | NT | |
| 11 | PERRY, ANDREW | Sullivan East High School | 2:22.74 | Verified |
| 12 | WINEGAR, LUKE | Volunteer High School | 2:26.01 | Verified |
| 13 | ELLISON, DUSTIN | Sullivan East High School | 2:26.55 | Verified |
| 14 | COX, JOE | Sullivan North High School | 2:26.80 | Verified |
| 15 | CLARK, SETH | Happy Valley High School | 2:27.36 | Verified |
| 16 | FERGUSON, LOGAN | Volunteer High School | 2:27.48 | Verified |
| 17 | DUNFORD, MATTHEW | Sullivan Central High School | 2:33.82 | Verified |
| 18 | LITTLEJOHN, LANCE | Sullivan East High School | 2:34.03 | Verified |
| 19 | FOSTER, JESSE | Cherokee High School | 2:34.69 | Verified |
| 20 | HUGHES, JORDAN | Cherokee High School | 2:34.72 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HARBAUGH, JOHN | Sullivan East High School | 2:35.00 | Unverified: |
| 2 | BABB, SPENCER | Happy Valley High School | 2:37.25 | Verified |
| 3 | TRIVETTE, BRYSON | Sullivan East High School | 2:38.29 | Verified |
| 4 | FORTENER, TAYLOR | Johnson Co. High School | 2:42.57 | Verified |
| 5 | WINTERS, ZEB | Sullivan North High School | 2:43.92 | Verified |
| 6 | BRADSHAW, JACOB | Sullivan East High School | 2:45.62 | Verified |
| 7 | ALMEN, JONATHAN | Sullivan South High School | 2:47.92 | Verified |
| 8 | ARMSTRONG, AYDAN | Happy Valley High School | 2:48.20 | Verified |
| 9 | CALDWELL, DAKOTA | Volunteer High School | 2:49.41 | Verified |
| 10 | HAYNES, ETHAN | Sullivan East High School | 2:50.70 | Verified |
| 11 | HUTCHINS, TY | Sullivan South High School | 2:55.18 | Verified |
| 12 | SLAGLE, ISAAC | Happy Valley High School | 2:58.29 | Verified |
| 13 | GILLESPIE, NOAH | Volunteer High School | 2:59.41 | Verified |
| 14 | HAYNES, CARSON | Sullivan East High School | 2:59.88 | Verified |
| 15 | ADKINSON, DAMON | Sullivan North High School | 3:01.00 | Unverified: |
| 16 | LOWE, TIMOTHY | Sullivan East High School | 3:16.31 | Verified |
| 17 | DOMINGUEZ, BRANNON | Johnson Co. High School | 3:20.02 | Verified |
| 18 | COATES, ELI | Sullivan South High School | NT | Unverified: |
| 19 | KINNISON, MICHAEL | Johnson Co. High School | NT | Unverified: |
| 20 | NOCERINO, NICK | Cherokee High School | NT | Unverified: |
| 21 | KUBACKI, ETHAN | Dobyns Bennett High School | NT | |
| 22 | CROW, ETHAN | Dobyns Bennett High School | NT |