| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ENSLEY, ELI | Sullivan East High School | 56.04 | Verified |
| 2 | SHORT, CORBIN | Volunteer High School | 55.80 | Unverified: |
| 3 | MAYS, TYLER | Dobyns Bennett High School | 54.00 | Unverified: |
| 4 | LEE, KAMDYN | Dobyns Bennett High School | 53.30 | Unverified: |
| 5 | MURPH, ANDREW | Volunteer High School | 51.58 | Verified |
| 6 | LOWE, TREY | Sullivan East High School | 51.97 | Verified |
| 7 | KIRKPATRICK, AUSTIN | Cherokee High School | 53.61 | Verified |
| 8 | SHORT, MASON | Volunteer High School | 55.80 | Unverified: |
| 9 | VAUGHN, CALEB | Sullivan South High School | 55.84 | Verified |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | HUGHES, JORDAN | Cherokee High School | 1:06.29 | Verified |
| 3 | NOCERINO, NICK | Cherokee High School | 1:01.73 | Verified |
| 4 | STRICKLAND, CHRISTOPHER | Volunteer High School | 58.01 | Verified |
| 5 | BLYTHE, GREYSON | Dobyns Bennett High School | 57.28 | Verified |
| 6 | LOCKLEAR, LANDEN | Sullivan Central High School | 57.62 | Verified |
| 7 | EARP, DAWSON | Sullivan East High School | 59.34 | Verified |
| 8 | FOSTER, JESSE | Cherokee High School | 1:05.36 | Verified |
| 9 | KINSLER, CODY | Sullivan North High School | 1:07.60 | Verified |