| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Science Hill High School | D | NT | |
| 2 | Daniel Boone High School | B | 48.81 | Unverified: Associated Orthopedics Big 11 Co |
| 3 | Happy Valley High School | A | 47.00 | Verified |
| 4 | Science Hill High School | C | 43.16 | Verified |
| 5 | Science Hill High School | B | 43.16 | Verified |
| 6 | Science Hill High School | A | 43.16 | Verified |
| 7 | Daniel Boone High School | A | 44.81 | Verified |
| 8 | University School of Johnson Cit | A | 47.72 | Verified |
| 9 | David Crockett High School | A | 59.18 | Verified |