| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Fort Dorchester | A | 3:36.47 | Last Chance Trojan Classic |
| 3 | Summerville | A | 3:31.72 | Dorchester County |
| 4 | Ashley Ridge | A | 3:30.18 | Tri County Meet of Champions |
| 5 | West Ashley | A | 3:31.39 | Tri County Meet of Champions |
| 6 | James Island | A | 3:34.90 | Charleston County School Distric |
| 7 | Stall | A | 3:49.18 | Last Chance Trojan Classic |