| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Shining Rock Classical Academy HS | A | NT | |
| 3 | Smoky Mountain | B | 47.06 | Pisgah Invitational @ UNCA |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | East Henderson | A | 46.27 | 20th Annual FCA Relays |
| 2 | Tuscola | A | 45.49 | McDowell High Meet #2 |
| 3 | Pisgah | A | 44.16 | WHKP Inv |
| 4 | Owen, Charles D. | A | DNS | UNC Asheville High School Classi |
| 5 | Hendersonville | A | 46.09 | WHKP Inv |
| 6 | Smoky Mountain | A | 47.06 | Pisgah Invitational @ UNCA |