DECLARE BY 12:55
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Roanoke | A | DNF | HPU VertKlasse Meeting |
| 2 | Bridgewater (Va.) | A | 42.37 | Doc Jopson Invitational |
| 3 | Winthrop | A | 42.04 | Big South Conference Outdoor Track and Field Championships |
| 4 | Louisburg College | A | 42.01 | The Adidas Trojan Challenge |
| 5 | Virginia State | A | 40.65 | Florida Relays |
| 6 | Louisburg College | B | 42.01 | The Adidas Trojan Challenge |
| 7 | Belmont Abbey | A | DNS | Montreat College Invitational |
| 8 | Pfeiffer | A | 42.56 | Atlantic Independent Track & Field Championship |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Randolph | A | 44.40 | Dr. Jack M. Toms Alumni Invitational |
| 4 | Guilford | A | 44.21 | W&L Track & Field Carnival |
| 5 | Louisburg College | C | 43.34 | The Adidas Trojan Challenge |
| 6 | Guilford | B | 44.21 | W&L Track & Field Carnival |