| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Belmont Abbey | A | Hicken, Genevieve | Caldwell, Ashley | Culbertson, Jillian | Otranto, Julia | 4:40.00 |
| 2 | High Point | A | Soper, CeAnna | Weisbrod, Anika | Reynolds, Madison | Wilbekin, Ariel | DNS |
| 3 | UNAT | A | Guess, Wynter | Black, Zariyah | Clemmons, Sydney | McLean, Corbin | 3:54.00 |
| 4 | Elon | A | Arbogast, Katherine | Henderson, Meagan | Savioz, Natacha | Brzozowski, Lauren | 3:43.32 |
| 5 | Virginia State | A | Frank, Kyla | Moseley, Alexandria | Bangura, Zainab | Green, Deriel | 3:53.00 |
| 6 | Elon | B | Langemann, Sarah | Varisco de Oliveira, Gabriela | Smith, Emily | King, Paige | DNS |
| 7 | Coker | A | Snelling, Charity | Ruff, Kaylah | Gale, Angel | Williamson, Jalecia | 4:12.00 |
| 8 | Washington and Lee | A | Smith, Claire | Cunningham, Anne | Fagan, Kathryn | Nelson, Anna | DNS |