| Athlete | Yr. | Team | Seed | |
|---|---|---|---|---|
| 1 | BLAIR, Allen | FR | Virginia State | 21.21 |
| 2 | MCELVEEN, jamel | JR | Platinum SportsAcademy | 21.65 |
| 3 | MURDOCK, Steven | JR | Virginia State | 21.66 |
| 4 | LILLY, Sage | FR | Coker | 21.80 |
| 5 | MCCRARY, Marquiz | SR | Platinum Sports | 21.85 |
| 6 | MCELVEEN, Tony | JR | Coker | 21.87 |
| 7 | FIORE, Donovan | SO | Washington and Lee | 21.90 |
| 8 | HOLMES, Montre | SO | Mount Olive | 21.93 |
| 9 | ATTUCKS, Cameron | SO | Platinum Sports Academy | 21.95 |
| 10 | WILLIAMS, Levi | FR | High Point | 21.99 |
| 11 | ANDERSON, Stephen | FR | Virginia State | 21.99 |
| 12 | CHERRY, Devante | JR | UNAT | 22.00 |
| 13 | GREENE, Erran | JR | Methodist | 22.00 |
| 14 | DOUGLAS, Montrell | FR | St. Andrews | 22.03 |
| 15 | ROMAN, Keshon | SO | Pfeiffer | 22.04 |
| 16 | PITTMAN, Brien | SR | UNAT | 22.10 |
| 17 | CARR, Trevor | SO | Mount Olive | 22.14 |
| 18 | JENKINS, Zach | FR | Mount Olive | 22.17 |
| 19 | PERRY, Montrell | FR | Mount Olive | 22.20 |
| 20 | SHANNON, Tedric | SO | Mount Olive | 22.25 |
| 21 | MUMFORD, Jarrel | SO | Coker | 22.29 |
| 22 | BULIK, Sven | FR | Mount Olive | 22.29 |
| 23 | MCDANIEL-BIDDINGS, Darius | JR | Mount Olive | 22.31 |
| 24 | FISHER, Richard | SO | Mount Olive | 22.36 |
| 25 | AUSTIN, Kameron | 8 | UNAT | 22.40 |
| 26 | SCOTT, Nihgel | JR | Barton | 22.43 |
| 27 | MCADAMS, Tylin | SR | KC Smoke TC | 22.46 |
| 28 | HUFFMAN, Carter | JR | Washington and Lee | 22.71 |
| 29 | JONES, Tim | SO | Mount Olive | 22.80 |
| 30 | WILSON, Micah | FR | Washington and Lee | 22.80 |
| 31 | WILKS, Collin | SR | Barton | 22.90 |
| 32 | CAINES, Alexander | FR | Washington and Lee | 23.00 |
| 33 | BARBOUR, Keith | FR | Virginia State | 23.18 |
| 34 | ALDRIDGE, Aaron | FR | Mount Olive | 23.26 |
| 35 | WIMBISH, Evan | FR | Catawba | 23.30 |
| 36 | CAMPBELL, Keyshawn | FR | Pfeiffer | 23.44 |
| 37 | DILLARD, Aspen | SO | Belmont Abbey | 23.66 |
| 38 | TAYLOR, Tony | SR | Methodist | 23.74 |
| 39 | JOHNSON, Jimmie | SO | Washington and Lee | 24.00 |
| 40 | KATTERHAGEN, Spencer | FR | Washington and Lee | 24.40 |
| 41 | COLEY, Kelvin | JR | Mount Olive | 24.55 |
| 42 | ABDUL-MATEEN, Ibraheem | FR | St. Andrews | 24.94 |