| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CHAPPELL, Alyssa | Volunteer High School | 29.90 |
| 2 | CUTSHALL, Caitlin | Daniel Boone High School | 29.18 |
| 3 | CHRISTIAN, Katie | Volunteer High School | 28.17 |
| 4 | PAGE, Macee | Dobyns Bennett High School | 27.98 |
| 5 | SALYERS, Ashley | Volunteer High School | 27.59 |
| 6 | DIXON, Taylor | Daniel Boone High School | 27.73 |
| 7 | HENGER, Aubree | Dobyns Bennett High School | 28.05 |
| 8 | BRISTOL, Injoi | Dobyns Bennett High School | 28.20 |
| 9 | FISH, Victoria | Dobyns Bennett High School | 29.88 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | STRYCHARZ, Suzanna | Sullivan South High School | 33.00 |
| 2 | OLMSTEAD, Kayeanna | Greeneville High School | 30.57 |
| 3 | BAGLEY, Ivy | Daniel Boone High School | 30.36 |
| 4 | ROSS, Lauren | Volunteer High School | 29.90 |
| 5 | CARDENAS, Lora | Volunteer High School | 29.90 |
| 6 | GIBSON, Payton | Volunteer High School | 29.90 |
| 7 | HAMILTON, Savanna | Volunteer High School | 29.96 |
| 8 | CARTER, Savannah | Daniel Boone High School | 30.48 |
| 9 | SPEARS, Taylor | Sullivan South High School | 33.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | CLEMONS, Maddie | Sullivan South High School | NT |
| 3 | BAILEY, Madison | Sullivan South High School | NT |
| 4 | SIMPSON, Kayla | Daniel Boone High School | NT |
| 5 | KERNEY, Arianna | Sullivan South High School | 33.42 |
| 6 | FRETWELL, Madison | Greeneville High School | NT |
| 7 | CLEAVER, Kaylee | Daniel Boone High School | NT |
| 8 | BRODIO, Mariyah | Daniel Boone High School | NT |
| 9 | HILTON, Aaliyah | Daniel Boone High School | NT |