| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Borghetti-Metz, Roman | SO | Volunteer High School | 2:03.99 | Verified |
| 2 | Wilson, Charlie | SR | Volunteer High School | 2:14.90 | Verified |
| 3 | Livesay, Isaiah | JR | Volunteer High School | 2:18.66 | Verified |
| 4 | Dawson, Evan | JR | Providence Academy | 2:23.36 | Verified |
| 5 | Ballard, Chase | 9 | Providence Academy | 2:26.16 | Verified |
| 6 | Armstrong, Connor | JR | Cherokee High School | 2:33.03 | Verified |
| 7 | Peterson, Nathan | SO | Cumberland Gap High School | 2:37.78 | Verified |
| 8 | Cochran, Lake | SR | Claiborne High School | 2:38.38 | Verified |
| 9 | Houston, Gavin | 9 | Cumberland Gap High School | 2:38.97 | Verified |
| 10 | Sattler, Holden | 9 | Cherokee High School | 2:41.60 | Verified |
| 11 | Munsey, Deacon | SO | Claiborne High School | 2:51.16 | Verified |
| 12 | Houck, Tucker | SR | Cherokee High School | 3:30.64 | Unverified: |