| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | Begley, Jacie | JR | Volunteer High School | 2:25.07 | Verified |
| 2 | Van Horn, Anna | JR | Providence Academy | 2:41.80 | Verified |
| 3 | Collins, Elliana | 9 | Providence Academy | 2:47.00 | Verified |
| 4 | Gibson, Chrissa | SO | Cumberland Gap High School | 2:51.22 | Verified |
| 5 | Vincent, Cami | JR | Cumberland Gap High School | 2:54.36 | Verified |
| 6 | Allen, Julina | 9 | Cherokee High School | 3:00.41 | Verified |
| 7 | Morton, Sophia | SO | Claiborne High School | 3:01.53 | Verified |
| 8 | Jones, Anna | SR | Claiborne High School | 3:03.00 | Verified |
| 9 | Russell, Landry | SR | Cherokee High School | 3:05.00 | Unverified: |
| 10 | Taylor, Sophia | SO | West Greene High School | 3:05.39 | Verified |
| 11 | Solomon, Hailey | JR | West Greene High School | 3:09.53 | Verified |
| 12 | Brooks, Hannah | JR | West Greene High School | 3:21.71 | Verified |